› 3f3r

X-ray diffraction
1.8Å resolution

Crystal structure of yeast Thioredoxin1-glutathione mixed disulfide complex

Released:
DOI: 10.2210/pdb3f3r/pdb
PDB entry 3f3r coloured by chain, front view.
PDB entry 3f3r coloured by chain, side view.
PDB entry 3f3r coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural and kinetic analysis of Saccharomyces cerevisiae thioredoxin Trx1: implications for the catalytic mechanism of GSSG reduced by the thioredoxin system.
Bao R, Zhang Y, Lou X, Zhou CZ, Chen Y
Biochim Biophys Acta 1794 1218-23 (2009)
PMID: 19362171

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149546 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 12.06 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22217 (Residues: 1-103; Coverage: 100%)
Gene names: TRX1, TRX2, YLR043C
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand GSH 2 x GSH
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 38.53Å b: 38.81Å c: 41.7Å
α: 72.91° β: 87.51° γ: 60.58°
R-values:
R R work R free
0.187 0.183 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Thioredoxin and glutaredoxin systems in plants: molecular mechanisms, crosstalks, and functional significance.
Meyer et al. (2012)
1 more

4 mentions without citation

Linked thioredoxin-glutathione systems in platyhelminth parasites: alternative pathways for glutathione reduction and deglutathionylation.
Bonilla et al. (2011)
3 more