› 3f3s

X-ray diffraction
2Å resolution

The Crystal Structure of Human Lambda-Crystallin, CRYL1

Released:
DOI: 10.2210/pdb3f3s/pdb
PDB entry 3f3s coloured by chain, front view.
PDB entry 3f3s coloured by chain, side view.
PDB entry 3f3s coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ugochukwu E, Johansson C, Yue WW, Kochan G, Pilka E, Kramm A, Pike ACW, Filippakopoulos P, von Delft F, Bountra C, Arrowsmith CH, Weigelt J, Edwards A, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
L-gulonate + NAD(+) = 3-dehydro-L-gulonate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-195153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lambda-crystallin homolog Chains: A, B
Molecule details ›
Chains: A, B
Length: 313 amino acids
Theoretical weight: 34.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y2S2 (Residues: 6-316; Coverage: 98%)
Gene names: CRY, CRYL1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
3 bound ligands:
Ligand SO4 4 x SO4
Ligand CL 4 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P65
Unit cell:
a: 102.085Å b: 102.085Å c: 134.986Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.154 0.196
Expression system: Escherichia coli

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