› 3f5b

X-ray diffraction
2Å resolution

The crystal structure of aminoglycoside N(6')acetyltransferase from Legionella pneumophila subsp. pneumophila str. Philadelphia 1.

Released:
DOI: 10.2210/pdb3f5b/pdb
PDB entry 3f5b coloured by chain, front view.
PDB entry 3f5b coloured by chain, side view.
PDB entry 3f5b coloured by chain, top view.
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Entry authors: Tan K, Wu R, Perez V, Jedrzejczak R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyltransferase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 182 amino acids
Theoretical weight: 21.4 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5ZWV6 (Residues: 1-179; Coverage: 100%)
Gene names: aacA4, lpg0979
Sequence domains: Acetyltransferase (GNAT) domain
Structure domains: Aminopeptidase

Ligands and Environments

5 bound ligands:
Ligand PO4 1 x PO4
Ligand ACT 1 x ACT
Ligand CHX 1 x CHX
Ligand FMT 4 x FMT
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 77.504Å b: 77.504Å c: 73.178Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.244
Expression system: Escherichia coli BL21

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