› 3f5f

X-ray diffraction
2.65Å resolution

Crystal structure of heparan sulfate 2-O-sulfotransferase from gallus gallus as a maltose binding protein fusion.

Released:
DOI: 10.2210/pdb3f5f/pdb
PDB entry 3f5f coloured by chain, front view.
PDB entry 3f5f coloured by chain, side view.
PDB entry 3f5f coloured by chain, top view.
Source organisms:
Primary publication:
Redirecting the substrate specificity of heparan sulfate 2-O-sulfotransferase by structurally guided mutagenesis.
Bethea HN, Xu D, Liu J, Pedersen LC
Proc Natl Acad Sci U S A 105 18724-9 (2008)
PMID: 19022906

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Heparan sulfate 2-O-sulfotransferase 1; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 658 amino acids
Theoretical weight: 74.29 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-387; Coverage: 98%)
  • Canonical: Q76KB1 (Residues: 69-356; Coverage: 81%)
Gene names: HS2ST, HS2ST1, JW3994, b4034, malE
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
1 bound ligand:
Ligand A3P 1 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P63
Unit cell:
a: 152.647Å b: 152.647Å c: 85.686Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.243
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Recent progress and applications in glycosaminoglycan and heparin research.
Laremore et al. (2009)
5 more

9 mentions without citation

A synergistic approach to protein crystallization: combination of a fixed-arm carrier with surface entropy reduction.
Moon et al. (2010)
8 more