3f5r

X-ray diffraction
1.7Å resolution

The crystal structure of a subunit of the heterodimeric FACT complex (Spt16p-Pob3p).

Released:
Source organism: Saccharomyces cerevisiae
Entry authors: Tan K, Xu X, Cui H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-170046 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit POB3 Chain: A
Molecule details ›
Chain: A
Length: 191 amino acids
Theoretical weight: 21.98 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04636 (Residues: 1-168; Coverage: 30%)
Gene names: POB3, YML069W
Sequence domains:
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3221
Unit cell:
a: 68.268Å b: 68.268Å c: 67.763Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.192
Expression system: Escherichia coli