› 3f7o

X-ray diffraction
2.2Å resolution

Crystal structure of Cuticle-Degrading Protease from Paecilomyces lilacinus (PL646)

Released:
DOI: 10.2210/pdb3f7o/pdb
PDB entry 3f7o coloured by chain, front view.
PDB entry 3f7o coloured by chain, side view.
PDB entry 3f7o coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structures of two cuticle-degrading proteases from nematophagous fungi and their contribution to infection against nematodes.
Liang L, Meng Z, Ye F, Yang J, Liu S, Sun Y, Guo Y, Mi Q, Huang X, Zou C, Rao Z, Lou Z, Zhang KQ
FASEB J 24 1391-400 (2010)
PMID: 20007510

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163537 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peptidase S8/S53 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 28.74 KDa
Source organism: Purpureocillium lilacinum
UniProt:
  • Canonical: Q01471 (Residues: 84-367; Coverage: 77%)
Sequence domains: Subtilase family
Structure domains: Peptidase S8/S53 domain
(MSU)(ALA)(ALA)(PRO)(VAL) Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 5 amino acids
Theoretical weight: 471 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 65.108Å b: 62.536Å c: 67.562Å
α: 90° β: 92.76° γ: 90°
R-values:
R R work R free
0.231 0.229 0.258
Expression system: Not provided

Quick links

Citations

7 review citations

Molecular mechanisms of nematode-nematophagous microbe interactions: basis for biological control of plant-parasitic nematodes.
Li et al. (2015)
6 more

5 mentions without citation

BioGPS descriptors for rational engineering of enzyme promiscuity and structure based bioinformatic analysis.
Ferrario et al. (2014)
4 more