› 3f8f

X-ray diffraction
2.2Å resolution

Crystal structure of multidrug binding transcriptional regulator LmrR complexed with Daunomycin

Released:
DOI: 10.2210/pdb3f8f/pdb
PDB entry 3f8f coloured by chain, front view.
PDB entry 3f8f coloured by chain, side view.
PDB entry 3f8f coloured by chain, top view.
Source organism: Lactococcus lactis subsp. cremoris MG1363
Primary publication:
Structure of the transcriptional regulator LmrR and its mechanism of multidrug recognition.
Madoori PK, Agustiandari H, Driessen AJ, Thunnissen AM
EMBO J 28 156-66 (2009)
PMID: 19096365

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator PadR N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 14.76 KDa
Source organism: Lactococcus lactis subsp. cremoris MG1363
Expression system: Lactococcus lactis
UniProt:
  • Canonical: A2RI36 (Residues: 1-116; Coverage: 100%)
Gene name: llmg_0323
Sequence domains: Transcriptional regulator PadR-like family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand DM1 1 x DM1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 35.45Å b: 53.025Å c: 147.116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.232 0.28
Expression system: Lactococcus lactis

Quick links

Citations

5 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
4 more

8 mentions without citation

Enabling protein-hosted organocatalytic transformations.
Nödling et al. (2020)
7 more