› 3f99

X-ray diffraction
1.65Å resolution

W354F Yersinia enterocolitica PTPase apo form

Released:
DOI: 10.2210/pdb3f99/pdb
PDB entry 3f99 coloured by chain, front view.
PDB entry 3f99 coloured by chain, side view.
PDB entry 3f99 coloured by chain, top view.
Source organism: Yersinia enterocolitica (type O:9)
Primary publication:
Impaired acid catalysis by mutation of a protein loop hinge residue in a YopH mutant revealed by crystal structures.
Brandão TA, Robinson H, Johnson SJ, Hengge AC
J Am Chem Soc 131 778-86 (2009)
PMID: 19140798

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase YopH Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 33.51 KDa
Source organism: Yersinia enterocolitica (type O:9)
Expression system: Escherichia coli
UniProt:
  • Canonical: P15273 (Residues: 164-468; Coverage: 65%)
Gene names: yop51, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 54.11Å b: 58.35Å c: 90.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.234
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Vanadium in Biological Action: Chemical, Pharmacological Aspects, and Metabolic Implications in Diabetes Mellitus.
Treviño et al. (2019)
5 more

2 mentions without citation

Impaired acid catalysis by mutation of a protein loop hinge residue in a YopH mutant revealed by crystal structures.
Brandão et al. (2009)
1 more