› 3f9t

X-ray diffraction
2.11Å resolution

Crystal structure of L-tyrosine decarboxylase MfnA (EC 4.1.1.25) (NP_247014.1) from METHANOCOCCUS JANNASCHII at 2.11 A resolution

Released:
DOI: 10.2210/pdb3f9t/pdb
PDB entry 3f9t coloured by chain, front view.
PDB entry 3f9t coloured by chain, side view.
PDB entry 3f9t coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reactions catalysed: 
L-aspartate = beta-alanine + CO(2)
L-tyrosine = tyramine + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178771 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-tyrosine/L-aspartate decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 45.6 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60358 (Residues: 1-396; Coverage: 100%)
Gene names: MJ0050, mfnA
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand MPD 3 x MPD
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 85.276Å b: 104.128Å c: 119.915Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.158 0.192
Expression system: Escherichia coli

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