› 3f9v

X-ray diffraction
4.35Å resolution

Crystal Structure Of A Near Full-Length Archaeal MCM: Functional Insights For An AAA+ Hexameric Helicase

Released:
DOI: 10.2210/pdb3f9v/pdb
PDB entry 3f9v coloured by chain, front view.
PDB entry 3f9v coloured by chain, side view.
PDB entry 3f9v coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Crystal structure of a near-full-length archaeal MCM: functional insights for an AAA+ hexameric helicase.
Brewster AS, Wang G, Yu X, Greenleaf WB, Carazo JM, Tjajadi M, Klein MG, Chen XS
Proc Natl Acad Sci U S A 105 20191-6 (2008)
PMID: 19073923

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194125 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Minichromosome maintenance protein MCM Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 67.32 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UXG1 (Residues: 7-601; Coverage: 87%)
Gene names: MCM, SSO0774
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I4122
Unit cell:
a: 202.526Å b: 202.526Å c: 128.243Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.415 0.412 0.481
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Mechanisms for initiating cellular DNA replication.
Costa et al. (2013)
22 more

28 mentions without citation

The Mcm complex: unwinding the mechanism of a replicative helicase.
Bochman et al. (2009)
27 more