› 3faw

X-ray diffraction
2.1Å resolution

Crystal Structure of the Group B Streptococcus Pullulanase SAP

Released:
DOI: 10.2210/pdb3faw/pdb
PDB entry 3faw coloured by chain, front view.
PDB entry 3faw coloured by chain, side view.
PDB entry 3faw coloured by chain, top view.
Source organism: Streptococcus agalactiae COH1
Primary publication:
Group B streptococcus pullulanase crystal structures in the context of a novel strategy for vaccine development.
Gourlay LJ, Santi I, Pezzicoli A, Grandi G, Soriani M, Bolognesi M
J Bacteriol 191 3544-52 (2009)
PMID: 19329633

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163541 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Reticulocyte binding protein Chain: A
Molecule details ›
Chain: A
Length: 877 amino acids
Theoretical weight: 98.56 KDa
Source organism: Streptococcus agalactiae COH1
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand CL 5 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 48.949Å b: 102.834Å c: 172.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.235
Expression system: Escherichia coli

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Citations

4 review citations

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
3 more

1 mention without citation

Group B streptococcus pullulanase crystal structures in the context of a novel strategy for vaccine development.
Gourlay et al. (2009)