› 3fax

X-ray diffraction
2.4Å resolution

The crystal structure of GBS pullulanase SAP in complex with maltotetraose

Released:
DOI: 10.2210/pdb3fax/pdb
PDB entry 3fax coloured by chain, front view.
PDB entry 3fax coloured by chain, side view.
PDB entry 3fax coloured by chain, top view.
Source organism: Streptococcus agalactiae COH1
Primary publication:
Group B streptococcus pullulanase crystal structures in the context of a novel strategy for vaccine development.
Gourlay LJ, Santi I, Pezzicoli A, Grandi G, Soriani M, Bolognesi M
J Bacteriol 191 3544-52 (2009)
PMID: 19329633

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Reticulocyte binding protein Chain: A
Molecule details ›
Chain: A
Length: 877 amino acids
Theoretical weight: 98.56 KDa
Source organism: Streptococcus agalactiae COH1
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
2 bound ligands:
Ligand CA 4 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 48.214Å b: 102.862Å c: 171.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.283
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
3 more

5 mentions without citation

Group B streptococcus pullulanase crystal structures in the context of a novel strategy for vaccine development.
Gourlay et al. (2009)
4 more