› 3fbd

X-ray diffraction
2.9Å resolution

Crystal structure of the nuclease domain of COLE7(D493Q mutant) in complex with an 18-BP duplex DNA

Released:
DOI: 10.2210/pdb3fbd/pdb
PDB entry 3fbd coloured by chain, front view.
PDB entry 3fbd coloured by chain, side view.
PDB entry 3fbd coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Wang YT, Doudeva LG, Yuan HS

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116243 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Colicin-E7 Chains: A, D
Molecule details ›
Chains: A, D
Length: 132 amino acids
Theoretical weight: 15.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 445-576; Coverage: 23%)
Gene names: cea, colE7
Sequence domains: Colicin/Pyocin-S2, DNase domain
Structure domains: Colicin/pyocin, DNase domain
5'-D(*DGP*DGP*DAP*DAP*DTP*DTP*DCP*DGP*DAP*DTP*DCP*DGP*DAP*DAP*DTP*DTP*DCP*DC)-3' Chains: B, C, E, F
Molecule details ›
Chains: B, C, E, F
Length: 18 nucleotides
Theoretical weight: 5.52 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 60.587Å b: 49.423Å c: 92.4Å
α: 90° β: 102.64° γ: 90°
R-values:
R R work R free
0.201 0.201 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 citation in other articles

A structural-alphabet-based strategy for finding structural motifs across protein families.
Wu et al. (2010)
6 more

1 mention without citation

Design of a colicin E7 based chimeric zinc-finger nuclease.
Németh et al. (2014)