3fbn

X-ray diffraction
3.01Å resolution

Structure of the Mediator submodule Med7N/31

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
PDBe Complex ID:
PDB-CPX-153690 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of RNA polymerase II transcription subunit 7 Chains: A, C
Molecule details ›
Chains: A, C
Length: 84 amino acids
Theoretical weight: 9.29 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08278 (Residues: 2-83; Coverage: 37%)
Gene names: MED7, YOL135C
Sequence domains: MED7 protein
Structure domains: Helix Hairpins
Mediator of RNA polymerase II transcription subunit 31 Chains: B, D
Molecule details ›
Chains: B, D
Length: 130 amino acids
Theoretical weight: 15.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P38633 (Residues: 1-127; Coverage: 100%)
Gene names: G2864, MED31, SOH1, YGL127C
Sequence domains: SOH1
Structure domains: Mediator of RNA polymerase II, submodule Med31 (Soh1)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: R32
Unit cell:
a: 174.62Å b: 174.62Å c: 117.84Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.178 0.231
Expression system: Escherichia coli