› 3fbr

X-ray diffraction
3.5Å resolution

structure of HipA-amppnp-peptide

Released:
DOI: 10.2210/pdb3fbr/pdb
PDB entry 3fbr coloured by chain, front view.
PDB entry 3fbr coloured by chain, side view.
PDB entry 3fbr coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Molecular mechanisms of HipA-mediated multidrug tolerance and its neutralization by HipB.
Schumacher MA, Piro KM, Xu W, Hansen S, Lewis K, Brennan RG
Science 323 396-401 (2009)
PMID: 19150849

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150091 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase toxin HipA Chain: A
Molecule details ›
Chain: A
Length: 437 amino acids
Theoretical weight: 49.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P23874 (Residues: 1-437; Coverage: 99%)
Gene names: JW1500, b1507, hipA
Sequence domains:
peptide of EF-Tu Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 784 Da
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: I422
Unit cell:
a: 128.4Å b: 128.4Å c: 203.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.282 0.282 0.336
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

71 review citations

Persister cells.
Lewis K. (2010)
70 more

5 mentions without citation

The secretory pathway kinases.
Sreelatha et al. (2015)
4 more