› 3fbt

X-ray diffraction
2.1Å resolution

Crystal structure of a chorismate mutase/shikimate 5-dehydrogenase fusion protein from Clostridium acetobutylicum

Released:
DOI: 10.2210/pdb3fbt/pdb
Model geometry
Fit model/data
PDB entry 3fbt coloured by chain, front view.
PDB entry 3fbt coloured by chain, side view.
PDB entry 3fbt coloured by chain, top view.
Source organism: Clostridium acetobutylicum
Entry authors: Bonanno JB, Gilmore M, Bain KT, Hu S, Romero R, Wasserman S, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Shikimate dehydrogenase (NADP(+)) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 282 amino acids
Theoretical weight: 31.72 KDa
Source organism: Clostridium acetobutylicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97KM0 (Residues: 97-367; Coverage: 74%)
Gene names: CA_C0897, aro, aroE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 65.132Å b: 135.336Å c: 67.14Å
α: 90° β: 102.14° γ: 90°
R-values:
R R work R free
0.236 0.233 0.299
Expression system: Escherichia coli

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