› 3fcc

X-ray diffraction
2.32Å resolution

CRYSTAL STRUCTURE OF DLTA PROTEIN IN COMPLEX WITH ATP and MAGNESIUM

Released:
DOI: 10.2210/pdb3fcc/pdb
PDB entry 3fcc coloured by chain, front view.
PDB entry 3fcc coloured by chain, side view.
PDB entry 3fcc coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Crystal structure of Bacillus cereus D-alanyl carrier protein ligase (DltA) in complex with ATP.
Osman KT, Du L, He Y, Luo Y
J Mol Biol 388 345-55 (2009)
PMID: 19324056

Function and Biology Details

Reaction catalysed: 
(1a) ATP + D-alanine = (D-alanyl)adenylate + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182523 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanyl carrier protein ligase Chain: A
Molecule details ›
Chain: A
Length: 512 amino acids
Theoretical weight: 57.63 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81G39 (Residues: 1-504; Coverage: 100%)
Gene names: BC_1372, dltA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P21
Unit cell:
a: 61.6Å b: 87Å c: 60.1Å
α: 90° β: 115° γ: 90°
R-values:
R R work R free
0.241 0.241 0.286
Expression system: Escherichia coli

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Citations

8 review citations

Lipoteichoic acid synthesis and function in gram-positive bacteria.
Percy et al. (2014)
7 more

5 mentions without citation

Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.
Gulick AM. (2009)
4 more