› 3fd2

X-ray diffraction
2.69Å resolution

Crystal structure of mMsoI/DNA complex with calcium

Released:
DOI: 10.2210/pdb3fd2/pdb
PDB entry 3fd2 coloured by chain, front view.
PDB entry 3fd2 coloured by chain, side view.
PDB entry 3fd2 coloured by chain, top view.
Source organism: Monomastix sp. OKE-1
Primary publication:
Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.
Li H, Pellenz S, Ulge U, Stoddard BL, Monnat RJ
Nucleic Acids Res 37 1650-62 (2009)
PMID: 19153140

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110993 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homing endonuclease LAGLIDADG domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 42.02 KDa
Source organism: Monomastix sp. OKE-1
Expression system: Escherichia coli
UniProt:
  • Canonical: C0JWR6 (Residues: 1-170; Coverage: 100%)
Gene name: orf170
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
5'-D(*GP*CP*AP*GP*AP*AP*CP*GP*TP*CP*GP*TP*GP*AP*GP*AP*CP*AP*GP*TP*TP*CP*CP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 24 nucleotides
Theoretical weight: 7.42 KDa
5'-D(*CP*GP*GP*AP*AP*CP*TP*GP*TP*CP*TP*CP*AP*CP*GP*AP*CP*GP*TP*TP*CP*TP*GP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.32 KDa

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P1
Unit cell:
a: 41.932Å b: 41.933Å c: 70.96Å
α: 107.21° β: 95.43° γ: 109.44°
R-values:
R R work R free
0.215 0.211 0.281
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
Silva et al. (2011)
10 more

1 mention without citation

Generation of single-chain LAGLIDADG homing endonucleases from native homodimeric precursor proteins.
Li et al. (2009)