› 3fd7

X-ray diffraction
1.53Å resolution

Crystal structure of Onconase C87A/C104A-ONC

Released:
DOI: 10.2210/pdb3fd7/pdb
PDB entry 3fd7 coloured by chain, front view.
PDB entry 3fd7 coloured by chain, side view.
PDB entry 3fd7 coloured by chain, top view.
Source organism: Lithobates pipiens
Primary publication:
Impact of the C-terminal disulfide bond on the folding and stability of onconase.
Schulenburg C, Weininger U, Neumann P, Meiselbach H, Stubbs MT, Sticht H, Balbach J, Ulbrich-Hofmann R, Arnold U
Chembiochem 11 978-86 (2010)
PMID: 20349493

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-149514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein P-30 Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.78 KDa
Source organism: Lithobates pipiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22069 (Residues: 1-104; Coverage: 100%)
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

3 bound ligands:
Ligand SO4 7 x SO4
Ligand GOL 3 x GOL
Ligand EDO 1 x EDO
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 45.46Å b: 51.19Å c: 87.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Emerging biological functions of ribonuclease 1 and angiogenin.
Garnett et al. (2022)
1 more

1 mention without citation

The CDI toxin of Yersinia kristensenii is a novel bacterial member of the RNase A superfamily.
Batot et al. (2017)