3fds

X-ray diffraction
2.05Å resolution

Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-157630 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase IV Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.26 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97W02 (Residues: 1-352; Coverage: 100%)
Gene names: SSO2448, dbh, dpo4
Sequence domains:
Structure domains:
DNA polymerase sliding clamp 1 Chain: C
Molecule details ›
Chain: C
Length: 249 amino acids
Theoretical weight: 27.52 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P57766 (Residues: 1-249; Coverage: 100%)
Gene names: C41_008, SSO0397, pcn1, pcnA-like
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 2 Chain: D
Molecule details ›
Chain: D
Length: 245 amino acids
Theoretical weight: 27.48 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Z84 (Residues: 1-244; Coverage: 100%)
Gene names: SSO1047, pcn2, pcnA-2
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 65.986Å b: 86.414Å c: 97.582Å
α: 90° β: 107.3° γ: 90°
R-values:
R R work R free
0.191 0.19 0.24
Expression system: Escherichia coli