› 3fef

X-ray diffraction
2.2Å resolution

Crystal structure of putative glucosidase lplD from bacillus subtilis

Released:
DOI: 10.2210/pdb3fef/pdb
PDB entry 3fef coloured by chain, front view.
PDB entry 3fef coloured by chain, side view.
PDB entry 3fef coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Ramagopal UA, Rajashankar KR, Toro R, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
((1->4)-alpha-D-galacturonide)(n) + H(2)O = ((1->4)-alpha-D-galacturonide)(n-1) + D-galacturonate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153839 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galacturonidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 450 amino acids
Theoretical weight: 50.52 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39130 (Residues: 7-446; Coverage: 99%)
Gene names: BSU07130, lplD
Sequence domains:
Structure domains: AglA-like glucosidase

Ligands and Environments

2 bound ligands:
Ligand SO4 7 x SO4
Ligand MG 4 x MG
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 81.987Å b: 84.843Å c: 86.051Å
α: 69.8° β: 68.1° γ: 64.07°
R-values:
R R work R free
0.19 0.187 0.243
Expression system: Escherichia coli

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