› 3few

X-ray diffraction
2.45Å resolution

Structure and Function of Colicin S4, a colicin with a duplicated receptor binding domain

Released:
DOI: 10.2210/pdb3few/pdb
PDB entry 3few coloured by chain, front view.
PDB entry 3few coloured by chain, side view.
PDB entry 3few coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and function of colicin S4, a colicin with a duplicated receptor-binding domain.
Arnold T, Zeth K, Linke D
J Biol Chem 284 6403-13 (2009)
PMID: 19056731

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194979 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Channel forming colicins domain-containing protein Chain: X
Molecule details ›
Chain: X
Length: 505 amino acids
Theoretical weight: 54.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XB47 (Residues: 1-499; Coverage: 100%)
Gene name: csa
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: R32
Unit cell:
a: 240.34Å b: 240.34Å c: 80.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.218 0.258
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Swimming against the tide: progress and challenges in our understanding of colicin translocation.
Kleanthous C. (2010)
6 more

10 mentions without citation

The Biology of Colicin M and Its Orthologs.
Chérier et al. (2021)
9 more