› 3ff5

X-ray diffraction
1.8Å resolution

Crystal structure of the conserved N-terminal domain of the peroxisomal matrix-protein-import receptor, Pex14p

Released:
DOI: 10.2210/pdb3ff5/pdb
PDB entry 3ff5 coloured by chain, front view.
PDB entry 3ff5 coloured by chain, side view.
PDB entry 3ff5 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Crystal structure of the conserved N-terminal domain of the peroxisomal matrix protein import receptor, Pex14p.
Su JR, Takeda K, Tamura S, Fujiki Y, Miki K
Proc Natl Acad Sci U S A 106 417-21 (2009)
PMID: 19122147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisomal membrane protein PEX14 Chains: A, B
Molecule details ›
Chains: A, B
Length: 54 amino acids
Theoretical weight: 6.07 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q642G4 (Residues: 25-70; Coverage: 12%)
Gene name: Pex14
Sequence domains: Pex14 N-terminal domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand DPW 1 x DPW
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: I23
Unit cell:
a: 90.57Å b: 90.57Å c: 90.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.211
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Getting a camel through the eye of a needle: the import of folded proteins by peroxisomes.
Lanyon-Hogg et al. (2010)
5 more

2 mentions without citation

A novel Pex14 protein-interacting site of human Pex5 is critical for matrix protein import into peroxisomes.
Neuhaus et al. (2014)
1 more