› 3ffy

X-ray diffraction
2Å resolution

Putative tetrapyrrole (corrin/porphyrin) methyltransferase from Bacteroides fragilis.

Released:
DOI: 10.2210/pdb3ffy/pdb
PDB entry 3ffy coloured by chain, front view.
PDB entry 3ffy coloured by chain, side view.
PDB entry 3ffy coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Osipiuk J, Volkart L, Cobb G, Kim Y, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytidine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(1402) in 16S rRNA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177505 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase I Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.06 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LHP3 (Residues: 112-224; Coverage: 50%)
Gene names: BF9343_0558, rsmI
Sequence domains: Tetrapyrrole (Corrin/Porphyrin) Methylases
Structure domains: Methyltransferase, Cobalt-precorrin-4 Transmethylase; Domain 2

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 60.536Å b: 60.536Å c: 96.065Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.231
Expression system: Escherichia coli

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