› 3fgg

X-ray diffraction
2.3Å resolution

Crystal Structure of Putative ECF-type Sigma Factor Negative Effector from Bacillus cereus

Released:
DOI: 10.2210/pdb3fgg/pdb
PDB entry 3fgg coloured by chain, front view.
PDB entry 3fgg coloured by chain, side view.
PDB entry 3fgg coloured by chain, top view.
Source organism: Bacillus cereus ATCC 10987
Entry authors: Kim Y, Nocek B, Maltseva N, Joachimiak G, Du J, Jedrzejczak R, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DUF3600 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 163 amino acids
Theoretical weight: 19.25 KDa
Source organism: Bacillus cereus ATCC 10987
Expression system: Escherichia coli
UniProt:
  • Canonical: Q739E5 (Residues: 67-226; Coverage: 71%)
Gene name: BCE_2196
Sequence domains: Domain of unknown function (DUF3600)
Structure domains: putative ecf-type sigma factor negative effector from bacillus cereus

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand GOL 6 x GOL
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 67.849Å b: 63.055Å c: 70.374Å
α: 90° β: 115.52° γ: 90°
R-values:
R R work R free
0.216 0.214 0.259
Expression system: Escherichia coli

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