› 3fgo

X-ray diffraction
2.5Å resolution

Crystal Structure of the E2 magnesium fluoride complex of the (SR) Ca2+-ATPase with bound CPA and AMPPCP

Released:
DOI: 10.2210/pdb3fgo/pdb
PDB entry 3fgo coloured by chain, front view.
PDB entry 3fgo coloured by chain, side view.
PDB entry 3fgo coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Cyclopiazonic acid is complexed to a divalent metal ion when bound to the sarcoplasmic reticulum Ca2+-ATPase.
Laursen M, Bublitz M, Moncoq K, Olesen C, Møller JV, Young HS, Nissen P, Morth JP
J Biol Chem 284 13513-13518 (2009)
PMID: 19289472

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 994 amino acids
Theoretical weight: 109.6 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P04191 (Residues: 1-993; Coverage: 99%)
Gene name: ATP2A1
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand MG 2 x MG
Ligand MF4 2 x MF4
Ligand K 2 x K
Ligand CZA 2 x CZA
Ligand MN 3 x MN
Ligand ACP 2 x ACP
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 101.427Å b: 108.872Å c: 274.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.217

Quick links

Citations

10 review citations

P-type ATPases.
Palmgren et al. (2011)
9 more

11 mentions without citation

Ion pathways in the sarcoplasmic reticulum Ca2+-ATPase.
Bublitz et al. (2013)
10 more