› 3fh6

X-ray diffraction
4.5Å resolution

Crystal structure of the resting state maltose transporter from E. coli

Released:
DOI: 10.2210/pdb3fh6/pdb
PDB entry 3fh6 coloured by chain, front view.
PDB entry 3fh6 coloured by chain, side view.
PDB entry 3fh6 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Alternating access in maltose transporter mediated by rigid-body rotations.
Khare D, Oldham ML, Orelle C, Davidson AL, Chen J
Mol Cell 33 528-36 (2009)
PMID: 19250913

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + maltose-[maltose-binding protein](Side 1) = ADP + phosphate + maltose(Side 2) + [maltose-binding protein](Side 1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-136375 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Maltose/maltodextrin transport system permease protein MalF Chains: F, H
Molecule details ›
Chains: F, H
Length: 480 amino acids
Theoretical weight: 53.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P02916 (Residues: 36-514; Coverage: 93%)
Gene names: JW3993, b4033, malF
Sequence domains:
Maltose/maltodextrin transport system permease protein MalG Chains: G, I
Molecule details ›
Chains: G, I
Length: 296 amino acids
Theoretical weight: 32.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68183 (Residues: 1-296; Coverage: 100%)
Gene names: JW3992, b4032, malG
Sequence domains: Binding-protein-dependent transport system inner membrane component
Maltose/maltodextrin import ATP-binding protein MalK Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 42.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P68187 (Residues: 1-371; Coverage: 100%)
Gene names: JW3995, b4035, malK
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: I222
Unit cell:
a: 171.097Å b: 209.48Å c: 438.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.34 0.34 0.363
Expression system: Escherichia coli

Quick links

Citations

35 review citations

A structural classification of substrate-binding proteins.
Berntsson et al. (2010)
34 more

28 mentions without citation

Lipid bilayer regulation of membrane protein function: gramicidin channels as molecular force probes.
Lundbaek et al. (2010)
27 more