› 3fhz

X-ray diffraction
3.27Å resolution

Crystal structure of the arginine repressor from Mycobacterium tuberculosis bound with its DNA operator and co-repressor, L-arginine

Released:
DOI: 10.2210/pdb3fhz/pdb
PDB entry 3fhz coloured by chain, front view.
PDB entry 3fhz coloured by chain, side view.
PDB entry 3fhz coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The structure of the arginine repressor from Mycobacterium tuberculosis bound with its DNA operator and Co-repressor, L-arginine.
Cherney LT, Cherney MM, Garen CR, James MN
J Mol Biol 388 85-97 (2009)
PMID: 19265706

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116247 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Arginine repressor Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 170 amino acids
Theoretical weight: 17.37 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPY9 (Residues: 1-170; Coverage: 100%)
Gene names: MTCY06H11.22, Rv1657, ahrC, argR
Sequence domains:
Structure domains:
5'-D(*TP*GP*TP*TP*GP*CP*AP*TP*AP*AP*CP*GP*AP*TP*GP*CP*AP*AP*AP*A)-3' Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided
5'-D(*TP*TP*TP*TP*GP*CP*AP*TP*CP*GP*TP*TP*AP*TP*GP*CP*AP*AP*CP*A)-3' Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand ARG 9 x ARG
Ligand ACT 2 x ACT
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 63.286Å b: 152.649Å c: 163.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.276
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
7 other articles

3 mentions without citation

Computer-based annotation of putative AraC/XylS-family transcription factors of known structure but unknown function.
Schüller et al. (2012)
2 more