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X-ray diffraction
2.3Å resolution

Crystal Structure of Leucine-bound LeuA from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb3fig/pdb
PDB entry 3fig coloured by chain, front view.
PDB entry 3fig coloured by chain, side view.
PDB entry 3fig coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structure of LeuA from Mycobacterium tuberculosis, a key enzyme in leucine biosynthesis.
Koon N, Squire CJ, Baker EN
Proc Natl Acad Sci U S A 101 8295-300 (2004)
PMID: 15159544

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-isopropylmalate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 646 amino acids
Theoretical weight: 70.29 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQB3 (Residues: 1-644; Coverage: 100%)
Gene names: MTV025.058, Rv3710, leuA
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand LEU 2 x LEU
Ligand GOL 2 x GOL
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 54.207Å b: 154.258Å c: 68.669Å
α: 90° β: 97.99° γ: 90°
R-values:
R R work R free
0.17 0.166 0.244
Expression system: Escherichia coli

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Citations

13 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
12 more

4 mentions without citation

Bacterial Branched-Chain Amino Acid Biosynthesis: Structures, Mechanisms, and Drugability.
Amorim Franco et al. (2017)
3 more