3fil

X-ray diffraction
0.88Å resolution

Structural and energetic determinants for hyperstable variants of GB1 obtained from in-vitro evolution

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-148792 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.25 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)
Immunoglobulin G-binding protein G Chain: B
Molecule details ›
Chain: B
Length: 56 amino acids
Theoretical weight: 6.22 KDa
Source organism: Streptococcus sp. 'group G'
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 303-357; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 29.694Å b: 38.93Å c: 83.326Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.125 0.124 0.149
Expression system: Escherichia coli BL21(DE3)