› 3fim

X-ray diffraction
2.55Å resolution

Crystal structure of aryl-alcohol-oxidase from Pleurotus eryingii

Released:
DOI: 10.2210/pdb3fim/pdb
PDB entry 3fim coloured by chain, front view.
PDB entry 3fim coloured by chain, side view.
PDB entry 3fim coloured by chain, top view.
Source organism: Pleurotus eryngii
Primary publication:
Novel structural features in the GMC family of oxidoreductases revealed by the crystal structure of fungal aryl-alcohol oxidase.
Fernández IS, Ruíz-Dueñas FJ, Santillana E, Ferreira P, Martínez MJ, Martínez AT, Romero A
Acta Crystallogr D Biol Crystallogr 65 1196-205 (2009)
PMID: 19923715

Function and Biology Details

Reaction catalysed: 
An aromatic primary alcohol + O(2) = an aromatic aldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 566 amino acids
Theoretical weight: 60.97 KDa
Source organism: Pleurotus eryngii
Expression system: Escherichia coli
UniProt:
  • Canonical: O94219 (Residues: 28-593; Coverage: 99%)
Gene name: aao
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16, EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P6422
Unit cell:
a: 180.226Å b: 180.226Å c: 160.407Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.206
Expression system: Escherichia coli

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Citations

3 review citations

Fungal aryl-alcohol oxidase: a peroxide-producing flavoenzyme involved in lignin degradation.
Hernández-Ortega et al. (2012)
2 more

22 mentions without citation

Human choline dehydrogenase: medical promises and biochemical challenges.
Salvi et al. (2013)
21 more