› 3fiu

X-ray diffraction
1.85Å resolution

Structure of NMN synthetase from Francisella tularensis

Released:
DOI: 10.2210/pdb3fiu/pdb
PDB entry 3fiu coloured by chain, front view.
PDB entry 3fiu coloured by chain, side view.
PDB entry 3fiu coloured by chain, top view.
Source organism: Francisella tularensis subsp. holarctica LVS
Primary publication:
Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis.
Sorci L, Martynowski D, Rodionov DA, Eyobo Y, Zogaj X, Klose KE, Nikolaev EV, Magni G, Zhang H, Osterman AL
Proc Natl Acad Sci U S A 106 3083-8 (2009)
PMID: 19204287

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163552 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 249 amino acids
Theoretical weight: 28.11 KDa
Source organism: Francisella tularensis subsp. holarctica LVS
Expression system: Escherichia coli
Structure domains: HUPs

Ligands and Environments

4 bound ligands:
Ligand MG 8 x MG
Ligand AMP 4 x AMP
Ligand POP 4 x POP
Ligand NA 4 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 50.432Å b: 126.438Å c: 152.531Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.233
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Microbial NAD metabolism: lessons from comparative genomics.
Gazzaniga et al. (2009)
5 more

3 mentions without citation

Nicotinamide mononucleotide synthetase is the key enzyme for an alternative route of NAD biosynthesis in Francisella tularensis.
Sorci et al. (2009)
2 more