› 3fkq

X-ray diffraction
2.1Å resolution

Crystal structure of NtrC-like two-domain protein (RER070207001320) from Eubacterium rectale at 2.10 A resolution

Released:
DOI: 10.2210/pdb3fkq/pdb
PDB entry 3fkq coloured by chain, front view.
PDB entry 3fkq coloured by chain, side view.
PDB entry 3fkq coloured by chain, top view.
Source organism: [Eubacterium] rectale
Primary publication:
Structure of the pilus assembly protein TadZ from Eubacterium rectale: implications for polar localization.
Xu Q, Christen B, Chiu HJ, Jaroszewski L, Klock HE, Knuth MW, Miller MD, Elsliger MA, Deacon AM, Godzik A, Lesley SA, Figurski DH, Shapiro L, Wilson IA
Mol Microbiol 83 712-27 (2012)
PMID: 22211578

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112191 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TadZ-like receiver domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 373 amino acids
Theoretical weight: 42.69 KDa
Source organism: [Eubacterium] rectale
Expression system: Escherichia coli
UniProt:
  • Canonical: D0VX10 (Residues: 1-354; Coverage: 100%)
Gene name: RER070207001320
Sequence domains: TadZ-like, receiver domain
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand 2PE 2 x 2PE
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 11 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2
Unit cell:
a: 121.506Å b: 79.495Å c: 55.022Å
α: 90° β: 96.16° γ: 90°
R-values:
R R work R free
0.191 0.19 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Pseudomonas aeruginosa twitching motility: type IV pili in action.
Burrows LL. (2012)
1 more

5 mentions without citation

Mechanistic characterization of GS-9190 (Tegobuvir), a novel nonnucleoside inhibitor of hepatitis C virus NS5B polymerase.
Shih et al. (2011)
4 more