› 3fku

X-ray diffraction
3.2Å resolution

Crystal structure of influenza hemagglutinin (H5) in complex with a broadly neutralizing antibody F10

Released:
DOI: 10.2210/pdb3fku/pdb
PDB entry 3fku coloured by chain, front view.
PDB entry 3fku coloured by chain, side view.
PDB entry 3fku coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses.
Sui J, Hwang WC, Perez S, Wei G, Aird D, Chen LM, Santelli E, Stec B, Cadwell G, Ali M, Wan H, Murakami A, Yammanuru A, Han T, Cox NJ, Bankston LA, Donis RO, Liddington RC, Marasco WA
Nat Struct Mol Biol 16 265-73 (2009)
PMID: 19234466

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212379 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 338 amino acids
Theoretical weight: 38.47 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6J8F6 (Residues: 17-346; Coverage: 62%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 182 amino acids
Theoretical weight: 20.98 KDa
Source organism: Influenza A virus (A/Viet Nam/1203/2004(H5N1))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6J8F6 (Residues: 347-522; Coverage: 33%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Neutralizing antibody F10 Chains: S, T, U, X, Y, Z
Molecule details ›
Chains: S, T, U, X, Y, Z
Length: 280 amino acids
Theoretical weight: 29.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: C2
Unit cell:
a: 205.3Å b: 118.5Å c: 338.9Å
α: 90° β: 99.6° γ: 90°
R-values:
R R work R free
0.235 0.232 0.286
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

217 review citations

Emergence and pandemic potential of swine-origin H1N1 influenza virus.
Neumann et al. (2009)
216 more

44 mentions without citation

Tackling influenza with broadly neutralizing antibodies.
Corti et al. (2017)
43 more