› 3fky

X-ray diffraction
2.95Å resolution

Crystal structure of the glutamine synthetase Gln1deltaN18 from the yeast Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb3fky/pdb
PDB entry 3fky coloured by chain, front view.
PDB entry 3fky coloured by chain, side view.
PDB entry 3fky coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of Saccharomyces cerevisiae glutamine synthetase Gln1 suggests a nanotube-like supramolecular assembly.
He YX, Gui L, Liu YZ, Du Y, Zhou Y, Li P, Zhou CZ
Proteins 76 249-54 (2009)
PMID: 19322816

Function and Biology Details

Reaction catalysed: 
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamine synthetase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T
Length: 370 amino acids
Theoretical weight: 41.76 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32288 (Residues: 1-370; Coverage: 100%)
Gene names: GLN1, YP3085.01, YP9367.15, YPR035W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FLC 20 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 128.65Å b: 129.94Å c: 135.61Å
α: 93.46° β: 104.61° γ: 104.01°
R-values:
R R work R free
0.226 0.225 0.258
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Glutamine synthetase in legumes: recent advances in enzyme structure and functional genomics.
Betti et al. (2012)
3 more

6 mentions without citation

Structures, functions, and mechanisms of filament forming enzymes: a renaissance of enzyme filamentation.
Park et al. (2019)
5 more