› 3flc

X-ray diffraction
1.85Å resolution

Crystal structure of the His-tagged H232R mutant of glycerol kinase from Enterococcus casseliflavus with glycerol

Released:
DOI: 10.2210/pdb3flc/pdb
PDB entry 3flc coloured by chain, front view.
PDB entry 3flc coloured by chain, side view.
PDB entry 3flc coloured by chain, top view.
Source organism: Enterococcus casseliflavus
Primary publication:
Structural characterizations of glycerol kinase: unraveling phosphorylation-induced long-range activation.
Yeh JI, Kettering R, Saxl R, Bourand A, Darbon E, Joly N, Briozzo P, Deutscher J
Biochemistry 48 346-56 (2009)
PMID: 19102629

Function and Biology Details

Reaction catalysed: 
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128519 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol kinase Chains: O, X
Molecule details ›
Chains: O, X
Length: 518 amino acids
Theoretical weight: 57.24 KDa
Source organism: Enterococcus casseliflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: O34153 (Residues: 1-506; Coverage: 100%)
Gene name: glpK
Sequence domains:
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

2 bound ligands:
Ligand GOL 2 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21212
Unit cell:
a: 92.559Å b: 192.234Å c: 57.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.233
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.
Deutscher et al. (2014)
1 more

2 mentions without citation

The FGGY carbohydrate kinase family: insights into the evolution of functional specificities.
Zhang et al. (2011)
1 more