› 3fmd

X-ray diffraction
2Å resolution

Crystal Structure of Human Haspin with an Isoquinoline ligand

Released:
DOI: 10.2210/pdb3fmd/pdb
PDB entry 3fmd coloured by chain, front view.
PDB entry 3fmd coloured by chain, side view.
PDB entry 3fmd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Filippakopoulos P, Eswaran J, Keates T, Burgess-Brown N, Fedorov O, Pike ACW, von Delft F, Arrowsmith CH, Edwards AM, Weigelt J, Bountra C, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase haspin Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TF76 (Residues: 470-798; Coverage: 41%)
Gene names: GSG2, HASPIN
Sequence domains: Haspin like kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand IQB 1 x IQB
Ligand NI 1 x NI
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E SUPERBRIGHT
Spacegroup: P212121
Unit cell:
a: 77.51Å b: 73.66Å c: 87.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.283
Expression system: Escherichia coli

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