› 3fme

X-ray diffraction
2.26Å resolution

Crystal Structure of Human Mitogen-Activated Protein Kinase Kinase 6 (MEK6) Activated Mutant (S207D, T211D)

Released:
DOI: 10.2210/pdb3fme/pdb
PDB entry 3fme coloured by chain, front view.
PDB entry 3fme coloured by chain, side view.
PDB entry 3fme coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Filippakopoulos P, Barr A, Pike ACW, Berridge G, Elkins J, Fedorov O, Keates T, Savitsky P, Soundararajan M, von Delft F, Arrowsmith CH, Edwards AM, Weigelt J, Bountra C, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity mitogen-activated protein kinase kinase 6 Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 32.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52564 (Residues: 47-334; Coverage: 86%)
Gene names: MAP2K6, MEK6, MKK6, PRKMK6, SKK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand STU 1 x STU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 132.756Å b: 132.756Å c: 45.719Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.213 0.212 0.236
Expression system: Escherichia coli

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