› 3fmg

X-ray diffraction
3.4Å resolution

Structure of rotavirus outer capsid protein VP7 trimer in complex with a neutralizing Fab

Released:
DOI: 10.2210/pdb3fmg/pdb
PDB entry 3fmg coloured by chain, front view.
PDB entry 3fmg coloured by chain, side view.
PDB entry 3fmg coloured by chain, top view.
Source organisms:
Primary publication:
Structure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.
Aoki ST, Settembre EC, Trask SD, Greenberg HB, Harrison SC, Dormitzer PR
Science 324 1444-7 (2009)
PMID: 19520960

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210523 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab of neutralizing antibody 4F8, light chain Chain: L
Molecule details ›
Chain: L
Length: 211 amino acids
Theoretical weight: 23.25 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab of neutralizing antibody 4F8, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.72 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Outer capsid glycoprotein VP7 Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.23 KDa
Source organism: Simian rotavirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12476 (Residues: 51-326; Coverage: 100%)
Sequence domains: Glycoprotein VP7
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P4132
Unit cell:
a: 244.179Å b: 244.179Å c: 244.179Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.238
Expression system: Spodoptera frugiperda

Quick links

Citations

16 review citations

Structural insights into the coupling of virion assembly and rotavirus replication.
Trask et al. (2012)
15 more

36 mentions without citation

Principles of virus structural organization.
Prasad et al. (2012)
35 more