› 3fmr

X-ray diffraction
2.89Å resolution

Crystal structure of an Encephalitozoon cuniculi methionine aminopeptidase type 2 with angiogenesis inhibitor TNP470 bound

Released:
DOI: 10.2210/pdb3fmr/pdb
PDB entry 3fmr coloured by chain, front view.
PDB entry 3fmr coloured by chain, side view.
PDB entry 3fmr coloured by chain, top view.
Source organism: Encephalitozoon cuniculi
Primary publication:
Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470.
Alvarado JJ, Nemkal A, Sauder JM, Russell M, Akiyoshi DE, Shi W, Almo SC, Weiss LM
Mol Biochem Parasitol 168 158-67 (2009)
PMID: 19660503

Function and Biology Details

Reaction catalysed: 
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186085 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine aminopeptidase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 358 amino acids
Theoretical weight: 40.03 KDa
Source organism: Encephalitozoon cuniculi
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8SR45 (Residues: 1-358; Coverage: 100%)
Gene names: ECU10_0750, MAP2
Sequence domains: Metallopeptidase family M24
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand TN4 2 x TN4
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 61.977Å b: 94.067Å c: 66.407Å
α: 90° β: 99.33° γ: 90°
R-values:
R R work R free
0.183 0.178 0.266
Expression system: Spodoptera frugiperda

Quick links

Citations

1 review citation

Metallo-aminopeptidase inhibitors.
Mucha et al. (2010)
12 other articles

1 mention without citation

Structure of a microsporidian methionine aminopeptidase type 2 complexed with fumagillin and TNP-470.
Alvarado et al. (2009)