› 3fms

X-ray diffraction
2.2Å resolution

Crystal structure of TM0439, a GntR transcriptional regulator

Released:
DOI: 10.2210/pdb3fms/pdb
PDB entry 3fms coloured by chain, front view.
PDB entry 3fms coloured by chain, side view.
PDB entry 3fms coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Primary publication:
Structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains.
Zheng M, Cooper DR, Grossoehme NE, Yu M, Hung LW, Cieslik M, Derewenda U, Lesley SA, Wilson IA, Giedroc DP, Derewenda ZS
Acta Crystallogr D Biol Crystallogr 65 356-65 (2009)
PMID: 19307717

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH gntR-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 24.51 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYS0 (Residues: 6-214; Coverage: 98%)
Gene name: TM_0439
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NI 1 x NI
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 85.188Å b: 71.718Å c: 43.323Å
α: 90° β: 104.59° γ: 90°
R-values:
R R work R free
0.161 0.157 0.228
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
1 more

6 mentions without citation

Structure of Thermotoga maritima TM0439: implications for the mechanism of bacterial GntR transcription regulators with Zn2+-binding FCD domains.
Zheng et al. (2009)
5 more