› 3fmt

X-ray diffraction
2.98Å resolution

Crystal structure of SeqA bound to DNA

Released:
DOI: 10.2210/pdb3fmt/pdb
PDB entry 3fmt coloured by chain, front view.
PDB entry 3fmt coloured by chain, side view.
PDB entry 3fmt coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural insights into the cooperative binding of SeqA to a tandem GATC repeat.
Chung YS, Brendler T, Austin S, Guarné A
Nucleic Acids Res 37 3143-52 (2009)
PMID: 19304745

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116249 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Negative modulator of initiation of replication Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 162 amino acids
Theoretical weight: 18.54 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFY8 (Residues: 1-181; Coverage: 90%)
Gene names: JW0674, b0687, seqA
Sequence domains:
Structure domains:
5'-D(*GP*AP*GP*TP*CP*GP*(6MA)P*TP*CP*GP*GP*CP*GP*GP*GP*(6MA)P*TP*CP*CP*TP*TP*A)-3' Chains: C, G
Molecule details ›
Chains: C, G
Length: 22 nucleotides
Theoretical weight: 6.84 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
5'-D(*TP*CP*TP*AP*AP*GP*GP*AP*TP*CP*CP*CP*GP*CP*CP*GP*AP*TP*CP*GP*AP*C)-3' Chains: D, H
Molecule details ›
Chains: D, H
Length: 22 nucleotides
Theoretical weight: 6.7 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MPD 4 x MPD
1 modified residue:
Ligand 6MA 4 x 6MA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: R3
Unit cell:
a: 121.839Å b: 121.839Å c: 279.133Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.218 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 citation in other articles

Dynamic distribution of seqa protein across the chromosome of escherichia coli K-12.
Sánchez-Romero et al. (2010)
11 more

1 mention without citation

Structural insights into the cooperative binding of SeqA to a tandem GATC repeat.
Chung et al. (2009)