› 3fmv

X-ray diffraction
3.31Å resolution

Crystal structure of the serine phosphatase of RNA polymerase II CTD (SSU72 superfamily) from Drosophila melanogaster. Monoclinic crystal form. Northeast Structural Genomics Consortium target FR253.

Released:
DOI: 10.2210/pdb3fmv/pdb
PDB entry 3fmv coloured by chain, front view.
PDB entry 3fmv coloured by chain, side view.
PDB entry 3fmv coloured by chain, top view.
Source organism: Drosophila melanogaster
Entry authors: Kuzin AP, Chen Y, Seetharaman J, Forouhar F, Chinag Y, Fang Y, Cunningham K, Ma L-C, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase II subunit A C-terminal domain phosphatase SSU72 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 195 amino acids
Theoretical weight: 23.33 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9VWE4 (Residues: 1-195; Coverage: 100%)
Gene names: BEST:CK01830, CG14216, Dmel\CG14216, Dmel_CG14216, Ssu72, dSsu72
Sequence domains: Ssu72-like protein
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 96 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 61.983Å b: 98.995Å c: 158.123Å
α: 90° β: 90.87° γ: 90°
R-values:
R R work R free
0.228 0.228 0.28
Expression system: Escherichia coli

Quick links