› 3fn0

X-ray diffraction
1.8Å resolution

Crystal structure of HIV-1 neutralizing human Fab Z13e1 in complex with a 12-residue peptide containing the Z13e1 epitope on gp41

Released:
DOI: 10.2210/pdb3fn0/pdb
PDB entry 3fn0 coloured by chain, front view.
PDB entry 3fn0 coloured by chain, side view.
PDB entry 3fn0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A conformational switch in human immunodeficiency virus gp41 revealed by the structures of overlapping epitopes recognized by neutralizing antibodies.
Pejchal R, Gach JS, Brunel FM, Cardoso RM, Stanfield RL, Dawson PE, Burton DR, Zwick MB, Wilson IA
J Virol 83 8451-62 (2009)
PMID: 19515770

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211623 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab Z13e1 Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Fab Z13e1 Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.75 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
Structure domains: Immunoglobulins
Envelope polyprotein gp160 Chain: P
Molecule details ›
Chain: P
Length: 9 amino acids
Theoretical weight: 1.22 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P41212
Unit cell:
a: 106.94Å b: 106.94Å c: 88.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.252
Expression system: Cricetulus griseus

Quick links

Citations

18 review citations

Human antibodies that neutralize HIV-1: identification, structures, and B cell ontogenies.
Kwong et al. (2012)
17 more

14 mentions without citation

Max Bergmann lecture protein epitope mimetics in the age of structural vaccinology.
Robinson JA. (2013)
13 more