› 3fn4

X-ray diffraction
1.96Å resolution

Apo-form of NAD-dependent formate dehydrogenase from bacterium Moraxella sp.C-1 in closed conformation

Released:
DOI: 10.2210/pdb3fn4/pdb
Model geometry
Fit model/data
PDB entry 3fn4 coloured by chain, front view.
PDB entry 3fn4 coloured by chain, side view.
PDB entry 3fn4 coloured by chain, top view.
Source organism: Moraxella sp.
Primary publication:
Structures of the apo and holo forms of formate dehydrogenase from the bacterium Moraxella sp. C-1: towards understanding the mechanism of the closure of the interdomain cleft.
Shabalin IG, Filippova EV, Polyakov KM, Sadykhov EG, Safonova TN, Tikhonova TV, Tishkov VI, Popov VO
Acta Crystallogr D Biol Crystallogr 65 1315-25 (2009)
PMID: 19966418

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126617 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 44.07 KDa
Source organism: Moraxella sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: O08375 (Residues: 2-402; Coverage: 100%)
Gene name: fdh
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: KURCHATOV SNC BEAMLINE K4.4
Spacegroup: C2
Unit cell:
a: 80.66Å b: 66.089Å c: 75.551Å
α: 90° β: 104.13° γ: 90°
R-values:
R R work R free
0.19 0.187 0.24
Expression system: Escherichia coli

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Citations

14 citation in other articles

Classification, substrate specificity and structural features of D-2-hydroxyacid dehydrogenases: 2HADH knowledgebase.
Matelska et al. (2018)
13 more