› 3fn9

X-ray diffraction
2.7Å resolution

Crystal structure of putative beta-galactosidase from bacteroides fragilis

Released:
DOI: 10.2210/pdb3fn9/pdb
PDB entry 3fn9 coloured by chain, front view.
PDB entry 3fn9 coloured by chain, side view.
PDB entry 3fn9 coloured by chain, top view.
Source organism: Bacteroides fragilis NCTC 9343
Entry authors: Ramagopal UA, Toro R, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-177516 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-galactosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 692 amino acids
Theoretical weight: 79.34 KDa
Source organism: Bacteroides fragilis NCTC 9343
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5LJ68 (Residues: 23-703; Coverage: 100%)
Gene name: BF9343_0029
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CL 7 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 109.453Å b: 133.066Å c: 221.192Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.27
Expression system: Escherichia coli

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