› 3fnd

X-ray diffraction
1.9Å resolution

Crystal structure of a chitinase from Bacteroides thetaiotaomicron

Released:
DOI: 10.2210/pdb3fnd/pdb
PDB entry 3fnd coloured by chain, front view.
PDB entry 3fnd coloured by chain, side view.
PDB entry 3fnd coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Entry authors: Damodharan L, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183676 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 36.73 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A3X9 (Residues: 47-347; Coverage: 93%)
Gene name: BT_2825
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 53.795Å b: 42.374Å c: 66.85Å
α: 90° β: 99.7° γ: 90°
R-values:
R R work R free
0.205 0.188 0.231
Expression system: Escherichia coli

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