› 3fop

X-ray diffraction
3Å resolution

A Triangular Cytochrome b562 Superstructure Mediated by Ni Coordination - Hexagonal Form

Released:
DOI: 10.2210/pdb3fop/pdb
PDB entry 3fop coloured by chain, front view.
PDB entry 3fop coloured by chain, side view.
PDB entry 3fop coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A superprotein triangle driven by nickel(II) coordination: exploiting non-natural metal ligands in protein self-assembly.
Radford RJ, Tezcan FA
J Am Chem Soc 131 9136-7 (2009)
PMID: 19527025

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Cytochrome c/b562

Ligands and Environments

3 bound ligands:
Ligand HEM 2 x HEM
Ligand PXX 2 x PXX
Ligand NI 4 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P6322
Unit cell:
a: 104.897Å b: 104.897Å c: 107.925Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.221 0.277
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Metal-directed protein self-assembly.
Salgado et al. (2010)
8 more

1 mention without citation

A superprotein triangle driven by nickel(II) coordination: exploiting non-natural metal ligands in protein self-assembly.
Radford et al. (2009)