› 3fpc

X-ray diffraction
1.4Å resolution

Chimera of alcohol dehydrogenase by exchange of the cofactor binding domain res 153-294 of T. brockii ADH by E. histolytica ADH

Released:
DOI: 10.2210/pdb3fpc/pdb
PDB entry 3fpc coloured by chain, front view.
PDB entry 3fpc coloured by chain, side view.
PDB entry 3fpc coloured by chain, top view.
Source organisms:
Primary publication:
Biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms.
Goihberg E, Peretz M, Tel-Or S, Dym O, Shimon L, Frolow F, Burstein Y
Biochemistry 49 1943-53 (2010)
PMID: 20102159

Function and Biology Details

Reaction catalysed: 
Propan-2-ol + NADP(+) = acetone + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADP-dependent isopropanol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 352 amino acids
Theoretical weight: 38.06 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P35630 (Residues: 7-9, 169-300; Coverage: 37%)
  • Canonical: P14941 (Residues: 4-162, 295-352; Coverage: 62%)
Gene names: ADH1, EHI_023110, adh
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand ZN 4 x ZN
Ligand CAC 4 x CAC
Ligand OXY 1 x OXY
Ligand EDO 14 x EDO
Ligand NO3 2 x NO3
Ligand IMD 1 x IMD
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 79.742Å b: 82.429Å c: 118.249Å
α: 90° β: 99.89° γ: 90°
R-values:
R R work R free
0.118 0.116 0.155
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains.
Collas et al. (2012)
1 more