› 3fpz

X-ray diffraction
1.82Å resolution

Saccharomyces cerevisiae THI4p is a suicide thiamin thiazole synthase

Released:
DOI: 10.2210/pdb3fpz/pdb
PDB entry 3fpz coloured by chain, front view.
PDB entry 3fpz coloured by chain, side view.
PDB entry 3fpz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase.
Chatterjee A, Abeydeera ND, Bale S, Pai PJ, Dorrestein PC, Russell DH, Ealick SE, Begley TP
Nature 478 542-6 (2011)
PMID: 22031445

Function and Biology Details

Reaction catalysed: 
NAD(+) + glycine + [ADP-thiazole synthase]-L-cysteine = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152255 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine thiazole synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 35 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32318 (Residues: 1-326; Coverage: 100%)
Gene names: ESP35, G6620, MOL1, THI4, YGR144W
Sequence domains: Thi4 family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AHZ 2 x AHZ
Ligand SO4 3 x SO4
1 modified residue:
Ligand DHA 2 x DHA

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I4
Unit cell:
a: 140.696Å b: 140.696Å c: 73.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.195 0.236
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Plant B vitamin pathways and their compartmentation: a guide for the perplexed.
Gerdes et al. (2012)
19 more

5 mentions without citation

Control of blood proteins by functional disulfide bonds.
Butera et al. (2014)
4 more